The use of radioactive isotopes in immunological investigations. 7. Labelled proteins and protein antigens containing both iodine and mustard gas sulphone groups.

PRODUCTION AND CHARACTERIZATION OF HUMORAL IMMUNE RESPONSE AGAINST MUSTARD GAS

One of the promising aspects of the immunological research on chemical war gas is to investigate the immunogenicity of some hazardous compounds such as mustard gas. Mustard gas is categorized as a "hapten" based on its physical and chemical properties. Haptenic chemicals which do not possess immunogenicity could be immunogenic experimentally when conjugated with a suitable protein carrier...

Prevention of Tryptophan Oxidation During Iodination of Tyrosyl Residues in Peptides

Introduction The conversion of tyrosyl residues into iodo-derivatives can be brought about under different ex­ perimental conditions [1 -3 ] and is essentially an electrophilic substitution by iodine at the phenolic ring of tyrosine. This reaction has been performed for different purposes in protein chemistry, e.g. the iodination of tyrosine has been extensively used in the preparation of label...

The use of radioactive isotopes in immunological investigations; fate of injected P32-containing proteins.

Comparative Study of Immunological and Structural Properties of Two Recombinant Vaccine Candidates against Botulinum Neurotoxin Type E

Background: Recently, botulinum neurotoxin (BoNT)-derived recombinant proteins have been suggested as potential botulism vaccines. Here, with concentrating on BoNT type E (BoNT/E), we studied two of these binding domain-based recombinant proteins: a multivalent chimer protein, which is composed of BoNT serotypes A, B and E binding subdomains, and a monovalent recombinant protein, which contains...

Labeling of Human Serum Albumin with Stable Isotope of Bromine; an in Vitro Study

Background: Possibility to trace-label albumin with isotopes results in information concerning its synthesis, breakdown, and distribution in the intra and extra cellular spaces. The iodination of albumin is a widespread procedure used in scientific studies. Bromine not only is more reactive and less expensive than iodine, but bonds more easily with many elements. Therefore, it could be a suitab...